Induction of Accelerated Acrosome Reaction in Guinea Pig Sperm1
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چکیده
منابع مشابه
Induction of the acrosome reaction in guinea pig spermatozoa by cGMP analogues
The effect of cyclic nucleotide analgoues upon the immediate induction of the guinea pig acrosome reaction (AR) was studied. Dibutyryl (dB) CGMP and 8-bromo-cGMP, when added to sperm suspensions after varying periods of preincubation in glucose-free BWW medium (NaCl 94.59 mM, KCl 4.7 mM, CaCl2 1.71 mM, KH2PO4 1.19 mM, MgSO4 1.19 mM, NaHCO3 25.07 mM, pyruvate 0.25 mM, lactate 21.58 mM, and bovin...
متن کاملThe induction of the acrosome reaction in guinea-pig sperm by the divalent metal cation ionophore A23187.
The divalent metal cation ionophore A23187 induces an acrosome reaction in guinea-pig sperm which is dependent on external calcium. Examination of this acrosome reaction by electron microscopy shows that it is morphologically normal. The known properties of A23187 and the morphological similarity between the acrosome reaction and the secretory discharges of other secretory cells suggests that t...
متن کاملThe activation of proteolysis in the acrosome reaction of guinea-pig sperm.
The divalent metal cation ionophore A23187 rapidly induces a normal acrosome reaction in a population of guinea-pig sperm suspended in calcium medium. In the course of the acrosome reaction, proacrosin, the zymogen precursor of the protease acrosin, is activated. Although the acrosome reaction causes exocytosis of the acrosomal contents, 'soluble' acrosin is not released in significant amounts ...
متن کاملThe histochemical localization of acrosin in guinea-pig sperm after the acrosome reaction.
The protease acrosin is widely considered to be an essential component of a zona lysin which enables sperm to penetrate the zona pellucida of the egg. Sperm form a characteristic penetration slit little wider than the sperm head itself and this has long suggested that any zona lysin is attached to the sperm surface after an acrosome reaction. This paper provides the first ultrastructural eviden...
متن کاملCapacitation-associated Changes in Protein-tyrosine-phosphorylation, Hyperactivation and Acrosome Reaction in Guinea Pig Sperm*
The aim of this study was to evaluate the effects of Ca, HCO3 and BSA on the in vitro capacitation-associated protein tyrosine phosphorylation, hyperactivation and acrosome reaction in guinea pig sperm. Caudal epididymal sperm were incubated in four different groups: modified TALP (Tyrode’s albumin lactate pyruvate) or TALP without one of the medium constituents (Ca, HCO3 and BSA). After incuba...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1980
ISSN: 0006-3363,1529-7268
DOI: 10.1093/biolreprod/22.3.566